commotiocordis (![[personal profile]](https://www.dreamwidth.org/img/silk/identity/user.png)
commotiocordis) wrote2009-04-08 04:46 pm
commotiocordis) wrote2009-04-08 04:46 pm
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You cannot define one of the "factors that influence pathogenicity" as "Virulence (degree of pathogenicity)". Seriously? That's like me saying "You know what influences how hungry I am? The degree of my hunger."
Also, things are not "adapt". They may be "adept," but fail.
/Microbiology teacher hate.
THINGS THAT ARE WIN, HERE THEY ARE. My E. coli guys. I transformed the Rad23 bit that I'm looking at into them with three different tagging vectors, and there's embedded in the swap-in bit for all three a gene for antibiotic resistance. That means that if it didn't transform the new DNA into the E. coli correctly, they wouldn't have the resistance so when I put them on the media plates with the antibiotic in the mix, they'd just be killed off. I had 6 batches, one of each of the tags plus a control for each of the tags with just the E. coli and all the other enzymes and such but without the Rad 23 segment inserted, and I went to check today and the ones that were supposed to grow grew and the ones that didn't, didn't. So win, especially considering I thought this stage had been ruined (and I would have had to go back several steps as I didn't have enough of the prepped R23 plasmid left over) when it was left incubating way too long at one stage because Dr. Smith left instructions to leave the instrumentation room unlocked, but there are two of those and so the wrong one was open and I couldn't get in to pull them out of the incubator.![[livejournal.com profile]](https://www.dreamwidth.org/img/external/lj-userinfo.gif)
bleakone and I (I dragged her along as we were on the way to the gym and it was only supposed to be a 15 minute stop of pulling the vials out of the incubator and putting them on the antibiotic plates and putting those back in the incubator) were in there for several hours as I tried to find a way to break in there. Turns out that in the evening on a Monday there's nobody in that building with a key. At all. I felt bad because then Dr. Smith had to come back in, but by the time he got my message, we had already given up and left, so he plated them for me.
Oh, and in news. US crew members have retaken their hijacked ship from the Somali pirates. That's right, suck it. I think we should fix our economy by becoming pirates, by the way. As a nation. It'd be awesome.
Also, things are not "adapt". They may be "adept," but fail.
/Microbiology teacher hate.
THINGS THAT ARE WIN, HERE THEY ARE. My E. coli guys. I transformed the Rad23 bit that I'm looking at into them with three different tagging vectors, and there's embedded in the swap-in bit for all three a gene for antibiotic resistance. That means that if it didn't transform the new DNA into the E. coli correctly, they wouldn't have the resistance so when I put them on the media plates with the antibiotic in the mix, they'd just be killed off. I had 6 batches, one of each of the tags plus a control for each of the tags with just the E. coli and all the other enzymes and such but without the Rad 23 segment inserted, and I went to check today and the ones that were supposed to grow grew and the ones that didn't, didn't. So win, especially considering I thought this stage had been ruined (and I would have had to go back several steps as I didn't have enough of the prepped R23 plasmid left over) when it was left incubating way too long at one stage because Dr. Smith left instructions to leave the instrumentation room unlocked, but there are two of those and so the wrong one was open and I couldn't get in to pull them out of the incubator.
bleakone and I (I dragged her along as we were on the way to the gym and it was only supposed to be a 15 minute stop of pulling the vials out of the incubator and putting them on the antibiotic plates and putting those back in the incubator) were in there for several hours as I tried to find a way to break in there. Turns out that in the evening on a Monday there's nobody in that building with a key. At all. I felt bad because then Dr. Smith had to come back in, but by the time he got my message, we had already given up and left, so he plated them for me. Oh, and in news. US crew members have retaken their hijacked ship from the Somali pirates. That's right, suck it. I think we should fix our economy by becoming pirates, by the way. As a nation. It'd be awesome.